Bacteriophages belong to viruses that only infect specific bacteria host. Phage is probably the most widely distributed biological entity in the biosphere, with an estimated population of > 10³⁰ of any environmental niche where bacteria or archaea reside. They play important roles in bacterial ecology, evolution, and pathogenesis of human bacterial infections. In our study, we use 40 different bacteriophages of the Gram-negative bacterium Escherichia coli as a model to find the rules behind bacteriophage genome organization and their general properties through comparative genomics analysis.

1. The phage genome offer an exciting opportunity to understand molecular mechanisms of phage diversity and evolution. On the other hand, the phage serve as potential therapeutic agents for antibiotic-resistant or no-antibiotic bacterial infection diseases, we hope that they can provide a new manner to improve clinical therapy and applications.
　

2.  Besides, current inefficient gene delivery represents a major obstacle for widespread use of promising approaches in molecular medicine, including human gene therapy. We will try to redesign competent phage vectors to increase the efficiency of gene delivery or gene therapy system with the obtained from the current study.